In vitro antiplasmodial activity and cytotoxicity of extracts and fractions of Vitex madiensis, medicinal plant of Gabon.
Identifieur interne : 001281 ( Main/Exploration ); précédent : 001280; suivant : 001282In vitro antiplasmodial activity and cytotoxicity of extracts and fractions of Vitex madiensis, medicinal plant of Gabon.
Auteurs : Joseph Privat Ondo [France] ; Jean-Bernard Lekana-Douki [Gabon] ; Jean-Bernard Bongui [Gabon] ; E. S. Zang Edou [Gabon] ; R. Zatra [Gabon] ; Fousseyni S. Toure-Ndouo [Gabon] ; Abdelhakim Elomri [France] ; J. Lebibi [Gabon] ; Elisabeth Seguin [France]Source :
- Tropical Medicine and International Health [ 1360-2276 ] ; 2012-03.
Abstract
Vitex madiensis Oliv. (Lamiaceae) is traditionally used to treat malaria symptoms in Haut-Ogooué, Gabon. Leaves and stem barks extracts were obtained using dichloromethane (CH(2)Cl(2)), ethyl acetate (EtOAc) and methanol (MeOH) as extraction solvents and fractionated on silica gel column. The in vitro antiplasmodial activity of CH(2)Cl(2), EtOAc and MeOH extracts and fractions was evaluated against the chloroquine-resistant FCB strain and field isolates of Plasmodium falciparum using the DELI test. The cytotoxicity of the extracts was tested on MRC-5 and THP1 cells using the tetrazolium salt MTT colorimetric assay, and the selectivity index (SI) of each extract was calculated. CH(2)Cl(2) extract, the EA1 fraction from EtOAc extract of stem barks and cyclohexane (L(cycl)), dichloromethane (L(DM)) and butanol (L(but)) fractions from MeOH/H(2)O extract of leaves exhibited the highest in vitro antiplasmodial activity on FCB strain and field isolates (IC(50) from 0.53 to 4.87 μg/ml) with high selectivity index (of 20.15-1800). These data support the use of V. madiensis in malaria treatment along with continued investigations within traditional medicines in the search of new antimalarial agents. The EA1, C(6)H(12) and CH(2)Cl(2) fractions could be selected for future investigation or/and for the treatment of malaria symptoms after standardization.
Url:
DOI: 10.1111/j.1365-3156.2011.02922.x
Affiliations:
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CS 14032 - 14032 Caen Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.normandie-univ.fr/</ref> </desc> </org></tutelle><tutelle active="#struct-301288" type="direct"><org type="institution" xml:id="struct-301288" status="VALID"> <idno type="IdRef">033364346</idno> <idno type="ISNI">0000000121908462</idno> <orgName>Institut national des sciences appliquées Rouen Normandie</orgName> <orgName type="acronym">INSA Rouen Normandie</orgName> <date type="start">1985-01-01</date> <desc> <address> <addrLine>Avenue de l’Université 76801 Saint-Étienne-du-Rouvray Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.insa-rouen.fr</ref> </desc> <listRelation> <relation active="#struct-455934" type="direct"></relation> <relation active="#struct-301232" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301232" type="indirect"><org type="regroupinstitution" xml:id="struct-301232" status="VALID"> <idno type="IdRef">162105150</idno> <orgName>Institut National des Sciences Appliquées</orgName> <orgName type="acronym">INSA</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Lekana Douki, Jean Bernard" sort="Lekana Douki, Jean Bernard" uniqKey="Lekana Douki J" first="Jean-Bernard" last="Lekana-Douki">Jean-Bernard Lekana-Douki</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-51216" status="VALID"> <orgName>Centre International de Recherches Médicales de Franceville</orgName> <orgName type="acronym">CIRMF</orgName> <desc> <address> <addrLine>Franceville</addrLine> <country key="GA"></country> </address> </desc> <listRelation> <relation active="#struct-301226" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301226" type="direct"><org type="institution" xml:id="struct-301226" status="VALID"> <orgName>Centre International de Recherches Médicales de Franceville</orgName> <desc> <address> <country key="GA"></country> </address> </desc> </org></tutelle></tutelles></hal:affiliation><country>Gabon</country></affiliation></author><author><name sortKey="Bongui, Jean Bernard" sort="Bongui, Jean Bernard" uniqKey="Bongui J" first="Jean-Bernard" last="Bongui">Jean-Bernard Bongui</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-377466" status="VALID"> <orgName>Université des Sciences et Techniques [Masuku]</orgName> <orgName type="acronym">USTM</orgName> <desc> <address> <addrLine>Franceville</addrLine> <country key="GA"></country> </address> <ref type="url">http://www.univ-masuku.ga/</ref> </desc> </hal:affiliation><country>Gabon</country></affiliation></author><author><name sortKey="Zang Edou, E S" sort="Zang Edou, E S" uniqKey="Zang Edou E" first="E. S." last="Zang Edou">E. S. Zang Edou</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-51216" status="VALID"> <orgName>Centre International de Recherches Médicales de Franceville</orgName> <orgName type="acronym">CIRMF</orgName> <desc> <address> <addrLine>Franceville</addrLine> <country key="GA"></country> </address> </desc> <listRelation> <relation active="#struct-301226" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301226" type="direct"><org type="institution" xml:id="struct-301226" status="VALID"> <orgName>Centre International de Recherches Médicales de Franceville</orgName> <desc> <address> <country key="GA"></country> </address> </desc> </org></tutelle></tutelles></hal:affiliation><country>Gabon</country></affiliation></author><author><name sortKey="Zatra, R" sort="Zatra, R" uniqKey="Zatra R" first="R." last="Zatra">R. 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CS 14032 - 14032 Caen Cedex 5</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.normandie-univ.fr/</ref> </desc> </org></tutelle><tutelle active="#struct-301288" type="direct"><org type="institution" xml:id="struct-301288" status="VALID"> <idno type="IdRef">033364346</idno> <idno type="ISNI">0000000121908462</idno> <orgName>Institut national des sciences appliquées Rouen Normandie</orgName> <orgName type="acronym">INSA Rouen Normandie</orgName> <date type="start">1985-01-01</date> <desc> <address> <addrLine>Avenue de l’Université 76801 Saint-Étienne-du-Rouvray Cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.insa-rouen.fr</ref> </desc> <listRelation> <relation active="#struct-455934" type="direct"></relation> <relation active="#struct-301232" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301232" type="indirect"><org type="regroupinstitution" xml:id="struct-301232" status="VALID"> <idno type="IdRef">162105150</idno> <orgName>Institut National des Sciences Appliquées</orgName> <orgName type="acronym">INSA</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"> <idno type="IdRef">02636817X</idno> <idno type="ISNI">0000000122597504</idno> <orgName>Centre National de la Recherche Scientifique</orgName> <orgName type="acronym">CNRS</orgName> <date type="start">1939-10-19</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.cnrs.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author></analytic><idno type="DOI">10.1111/j.1365-3156.2011.02922.x</idno><series><title level="j">Tropical Medicine and International Health</title><idno type="ISSN">1360-2276</idno><imprint><date type="datePub">2012-03</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>Vitex madiensis Oliv. (Lamiaceae) is traditionally used to treat malaria symptoms in Haut-Ogooué, Gabon. Leaves and stem barks extracts were obtained using dichloromethane (CH(2)Cl(2)), ethyl acetate (EtOAc) and methanol (MeOH) as extraction solvents and fractionated on silica gel column. The in vitro antiplasmodial activity of CH(2)Cl(2), EtOAc and MeOH extracts and fractions was evaluated against the chloroquine-resistant FCB strain and field isolates of Plasmodium falciparum using the DELI test. The cytotoxicity of the extracts was tested on MRC-5 and THP1 cells using the tetrazolium salt MTT colorimetric assay, and the selectivity index (SI) of each extract was calculated. CH(2)Cl(2) extract, the EA1 fraction from EtOAc extract of stem barks and cyclohexane (L(cycl)), dichloromethane (L(DM)) and butanol (L(but)) fractions from MeOH/H(2)O extract of leaves exhibited the highest in vitro antiplasmodial activity on FCB strain and field isolates (IC(50) from 0.53 to 4.87 μg/ml) with high selectivity index (of 20.15-1800). These data support the use of V. madiensis in malaria treatment along with continued investigations within traditional medicines in the search of new antimalarial agents. The EA1, C(6)H(12) and CH(2)Cl(2) fractions could be selected for future investigation or/and for the treatment of malaria symptoms after standardization.</p> </div></front></TEI><affiliations><list><country><li>France</li><li>Gabon</li></country></list><tree><country name="France"><noRegion><name sortKey="Ondo, Joseph Privat" sort="Ondo, Joseph Privat" uniqKey="Ondo J" first="Joseph Privat" last="Ondo">Joseph Privat Ondo</name></noRegion><name sortKey="Elomri, Abdelhakim" sort="Elomri, Abdelhakim" uniqKey="Elomri A" first="Abdelhakim" last="Elomri">Abdelhakim Elomri</name><name sortKey="Seguin, Elisabeth" sort="Seguin, Elisabeth" uniqKey="Seguin E" first="Elisabeth" last="Seguin">Elisabeth Seguin</name></country><country name="Gabon"><noRegion><name sortKey="Lekana Douki, Jean Bernard" sort="Lekana Douki, Jean Bernard" uniqKey="Lekana Douki J" first="Jean-Bernard" last="Lekana-Douki">Jean-Bernard Lekana-Douki</name></noRegion><name sortKey="Bongui, Jean Bernard" sort="Bongui, Jean Bernard" uniqKey="Bongui J" first="Jean-Bernard" last="Bongui">Jean-Bernard Bongui</name><name sortKey="Lebibi, J" sort="Lebibi, J" uniqKey="Lebibi J" first="J." last="Lebibi">J. Lebibi</name><name sortKey="Toure Ndouo, Fousseyni S" sort="Toure Ndouo, Fousseyni S" uniqKey="Toure Ndouo F" first="Fousseyni S." last="Toure-Ndouo">Fousseyni S. Toure-Ndouo</name><name sortKey="Zang Edou, E S" sort="Zang Edou, E S" uniqKey="Zang Edou E" first="E. S." last="Zang Edou">E. S. Zang Edou</name><name sortKey="Zatra, R" sort="Zatra, R" uniqKey="Zatra R" first="R." last="Zatra">R. Zatra</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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